Targeting the Zebrafish Optic Tectum Using In Vivo Electroporation
نویسندگان
چکیده
منابع مشابه
Layer-Specific Targeting of Direction-Selective Neurons in the Zebrafish Optic Tectum
Direction selectivity (DS) is an important neuronal property in the visual system, but how DS is generated beyond the retina remains controversial. Here, we report a close correspondence between the preferred direction (PD) and the morphology of DS cells in the optic tectum. Ca(2+) imaging in cells expressing the genetically encoded Ca(2+) indicator GCaMP3 and multiphoton-targeted patch-clamp r...
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Reverse correlation techniques provide a quantitative means of computing neuronal input/output relationships. Until now these methods have been limited to electrically recorded responses since unprocessed optical signals generally lack necessary temporal characteristics. We sought to overcome this barrier since combining reverse correlation with calcium imaging would afford a powerful alternati...
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The optic tectum of larval zebrafish is an important model for understanding visual processing in vertebrates. The tectum has been traditionally viewed as dominantly visual, with a majority of studies focusing on the processes by which tectal circuits receive and process retinally-derived visual information. Recently, a handful of studies have shown a much more complex role for the optic tectum...
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In vivo electroporation is a powerful method for delivering DNA expression plasmids, RNAi reagents, and morpholino anti-sense oligonucleotides to specific regions of developing embryos, including those of C. elegans, chick, Xenopus, zebrafish, and mouse. In zebrafish, in vivo electroporation has been shown to have excellent spatial and temporal resolution for the delivery of these reagents. The...
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The optic tectum forms the roof of the midbrain and is the primary visual center in fishes. The retina and input from other sensory modalities (auditory, lateral line, somatosensory, electrosensory) are mapped topographically over tectum, where the locations of objects and events in body-centered space are represented. Tectum, via its connections to premotor centers, controls eye movements, app...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2010
ISSN: 1559-6095
DOI: 10.1101/pdb.prot5463